Hemorrhagic Fever with Renal syndrome9HFRS0 is an acute infectious disease transmitted by Hantaan virus and related viruses, and is pathologically characterized as the destruction of endothelium of capilary and arterioles by direct invasion of
virus and
secondary damage following immune response. Soluble HLA class I antigen(sHLA-I) is normally present in serum and the level in serum is changed according to clinical status at various diseases. The role and the meaning of sHLA-I in serum are still
controversal and investigated. In vitro it is actively sereted by normal B and T lymphcytes when they are stimulated by appropriate mitogin (phytohemagglutinin, anti-CD3 antibody) and lymphokines (IFN-r, TNF).
To assess sHLA-I in serum and in urine from 21 normale healthy persons and 35 patient with HFRS, we established double-sandwich ELISA method using W6/32(anti-HLA class I monoclonal antibody0 for coating and HRP-conjugated
BBM1(anti-beta2-microglobin
monoclonal antibody) for detection. The titer of sHLA-I was expressed with arbitrary init(AU).
The sHLA-I in serum form patients with HFRS(415±224AU) was ignifically higher than those in normals(70±52AU(p<.0.05). During clinical course, the titers of sHLA-I was very high in febrile, hypotensive, and oliguric phase(382±122AU, 433±44AU
nad
423±173AU) and was slowly declined in following diuretic and convcvaleseent phase(318±142AU, and 190±10AU). The titer of anti-Hantaan virus antibody was increased later than soluble HLA class I antigen. The sHLA-I in urine of healthy persons
was
absent, however it was detected transiently at febrile and diuretic phases of HFRS(23AU and 5AU). These results suggest that the level of the sHLA-I in serum and urine well reflects clinical course and severity of HFRS.
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